Fig. 2. Prolidase and PRODH/POX expression (A) and prolidase activity (B) in wild type MCF-7 and MCF-7 transfected cells with prolidase cDNA expression vector (MCF-7PL). Representative Western immunoblot for prolidase of control and MCF-7 PL cells are presented. GAPDH was used as a reference protein. The mean values of bands densitometry were added above appropriated bands. For prolidase activity and Western blot analysis (Supplementary data - see www.cellphysiolbiochem.com) the mean values for 6 assays + S.D were calculated and submitted to statistical analysis using the ANOVA test, accepting P<0.001 as significant.